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KMID : 0376219830200010223
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Chonnam Medical Journal
1983 Volume.20 No. 1 p.223 ~ p.236
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The enzyme histochemical studies on the timing of wound
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Abstract
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In postmortem examination, it is often a difficult task to estimate the elapsed time between the initial injury and subsequent death. Therefore, in order to estimate the timing of wound, an enzyme histochemical studies of various enzymes were carried out on rats, which had experimentally inflicted skin wounds, at sequential intervals for 10 days after death.
1. In the peripheral zone of the wound, there was a gradual increase in enzyme activity. This was demonstrable from the following times on; a-naphthyl acetate esterase(ANAE), monoamine oxidase(MAO) and b-glucuronidase activities 1 hour, adenosine triphosphatase(ATPase) and leucine aminopeptidase(LAP) activities 2 hours, acid and alkaline phosphatase activities 4 hours, and succinic dehydrogenase(SDH) activity 8 hours after the injury.
2. An intense or morderate increase in enzyme activity, which persisted throught the experimental period was seen from following times on; acid phohatase, a-naphthyl acetate esterase(ANAE) and b-glucuronidase activities 8 hours, alkaline phosphatase and leucine aminopeptidase(LAP) activities 16 hours after wounding. However, the following enzymes should limited increase in activity up to the indicated times; adenosine triphosphatase(ATPase) 16 hours, succinic dehydrogenase(SDH) 1day and monoamine oxidase(MAO) activities 8 hours.
3. The increased enzyme activities were fairly well preserved after death for the following times on; monoamine oxidase(MAO) and adenosine triphosphatase (ATPase) activities 2 days, succinct dehydrogenate(SDH) activity 3 days, acid phosphatase and leucine aminopeptidase(LAP) activities 5 days, alkaline phosphatase 7 days, a-naphthyl acetate esterase(ANAE) and b-glucuronidase activities for 10 days afterwards.
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